ABSTRACT
BACKGROUND: The most common problem in HLA typing is unsatisfactory quality of the antisera, or a lack of understanding of their reactivities. Therefore, commercial antisera must be verified under the conditions applied in a particular tissue typing laboratory. METHODS: We evaluated the antisera reactivities of a commercial HLA-yping tray, Lymphotype HLA-BC 72 oriental, the lot 7220999, 7230100 (Biotest, Germany), in about 300 samples from organ transplant recipients and healthy potential donors. RESULTS: The relatively weak antisera were those that defined A26, A33, Cw5, Cw14, B46, B58, B64 and B71 etc. Some of these antisera were not indicated as 'weak reaction' in the test result catalogue. The reactivities of each antisera indicated as 'extra reaction' or 'sometimes missing' were various. CONCLUSIONS: As for antisera reactivities, the data obtained by a laboratory itself are necessary in addition to those in the test result catalogue. These data will be helpful for the correct interpretation for laboratories using same commercial kits.
Subject(s)
Humans , Histocompatibility Testing , Immune Sera , Tissue Donors , TransplantsABSTRACT
BACKGROUND: The most common problem in HLA typing is unsatisfactory quality of the antisera, or a lack of understanding of their reactivities. Therefore, commercial antisera must be verified under the conditions applied in a particular tissue typing laboratory. METHODS: We evaluated the antisera reactivities of a commercial HLA-yping tray, Lymphotype HLA-BC 72 oriental, the lot 7220999, 7230100 (Biotest, Germany), in about 300 samples from organ transplant recipients and healthy potential donors. RESULTS: The relatively weak antisera were those that defined A26, A33, Cw5, Cw14, B46, B58, B64 and B71 etc. Some of these antisera were not indicated as 'weak reaction' in the test result catalogue. The reactivities of each antisera indicated as 'extra reaction' or 'sometimes missing' were various. CONCLUSIONS: As for antisera reactivities, the data obtained by a laboratory itself are necessary in addition to those in the test result catalogue. These data will be helpful for the correct interpretation for laboratories using same commercial kits.